Functional identification of effectors of fusion proteins that drive secondary-type acute myeloid leukaemia (WP2)
Rationale and objectives
Fusion proteins often act as strong drivers of hematological malignancies. We have developed mouse models and cell line tools for the mechanistic investigation of NUP98-fusion proteins, which are found in myelodysplastic syndromes (MDS) and seconday Acute Myeloid Luekaemia (sAML), including NUP98-HOXA9, NUP98-NSD1 and NUP98-DDX10.
The project's objectives are:
• To generate, analyze and integrate data sets of transcriptomics, functional genomics or proteomics datasets.
• To test the role of candidate regulators (50-100) using targeted CRISPR/Cas9 loss-of-function genetics.
• To investigate the mechanistic basis of 1-3 high-confidence candidates at the single-cell level using complementary approaches.
Institut national de la sante et de la recherche medicale (France, 4 months), GenomeScan B.V. (The Netherlands, 2 months).
PhD Programme of the Vetmeduni Vienna, Austria.