Cross-talk between single haematopoietic stem/progenitor cells and bone marrow niche in myelodysplastic syndromes and secondary-type acute myeloid leukaemia (WP1)
Rationale and objectives
In disease and aging, the stroma cells of the bone marrow are epigenetically altered and support less healthy haematopoietic stem/progenitor cells (HSPCs). Whether this decline is cell intrinsic or instructed by mutated HSPCs is not fully understood.
Our objectives are:
• To analyze on the single-cell level the reciprocal influence of HSPCs and stroma cells on gene transcription.
• To identify and validate gene signatures correlating with MDS and normal aging.
• To functionally test involved key pathways in vitro and in vivo using CRISPR/CAS9-engineered cells.
GenomeScan B.V. (The Netherlands, 3 months), Institute for Tumor Biology and Experimental Therapy, Georg-Speyer-Haus (Germany, 3 months).
PhD in Experimental Medicine, TUM graduate School (TUM-GS), Germany.